Optimized 3D imaging of biological objects by X-ray microtomography methods

Optimized X-ray microtomographic methodologies of three-dimensional visualization of biological objects and their internal microstructures were developed. Optimization of microtomographic measurements for a specific object included the choice of target material, optimized acceleration voltage and X-ray tube current, signal accumulation time on the detector, number of repetitions, choice of relaxation time between two measurements and number of X-ray projections. An important part of optimized imaging is the area of digital processing of microtomographic measurement results and their visualization, where methods of data filtering, rendering and image segmentation have been developed.
The methodologies were used in cooperation with Prof. J. Klembar from PriF UK in a comparative anatomical study of the skull of legless lizards Pseudopus Apodus [1] and Dr. ¼. A fork from the Institute of Zoology of the Slovak Academy of Sciences in the description of the anatomy of a newly discovered cave cockroach [2].

 

Publications:

1. KLEMBARA, J. – DOBIAŠOVÁ, K. – HAIN, M. – YARYHIN, O. Skull anatomy and ontogeny of legless lizard Pseudopus apodus (Pallas, 1775): Heterochronic influences on form. In The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology, 2017, vol. 300, no. 3, p. 460-502.
2. VIDLIČKA, Ľ. – VRŠANSKÝ, P. – KÚDELOVÁ, T. – KÚDELA, M. – DEHARVENG, L. – HAIN, M. New genus and species of cavernicolous cockroach (Blattaria, Nocticolidae) from Vietnam. In Zootaxa, 2017, vol. 4232, no. 3, p. 361-375.